Full structure of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 5

Biochemistry (Mosc). 2004 Feb;69(2):170-5. doi: 10.1023/b:biry.0000018947.60328.8d.

Abstract

The lipopolysaccharide (LPS) of the opportunistic human pathogen Pseudomonas aeruginosa immunotype 5 was delipidated by mild acid hydrolysis, and the products were separated by high-performance anion-exchange chromatography and analyzed by ESI MS and NMR spectroscopy. LPS species of three types were found, including those with an unsubstituted core and the core substituted with one O-polysaccharide repeating unit or with an O-polysaccharide of a variable number of repeating units. The core region is highly phosphorylated, the major species containing two monophosphate groups and one ethanolamine diphosphate group. Based on these and published data on the O-polysaccharide structure, the full structure of the LPS of P. aeruginosa immunotype 5 was established.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Bacterial / chemistry*
  • Antigens, Bacterial / immunology
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / immunology
  • Magnetic Resonance Spectroscopy
  • Oligosaccharides / chemistry*
  • Pseudomonas aeruginosa / chemistry*
  • Pseudomonas aeruginosa / immunology

Substances

  • Antigens, Bacterial
  • Lipopolysaccharides
  • Oligosaccharides