IP-10, a gamma-interferon-inducible protein related to interleukin-8, lacks neutrophil activating properties

Immunol Lett. 1992 Mar;32(1):81-4. doi: 10.1016/0165-2478(92)90203-z.


IP-10, a small, gamma-interferon-inducible protein with structural homology to interleukin-8 (IL-8), was prepared by automated chemical synthesis and compared with synthetic IL-8, GRO alpha and neutrophil-activating peptide 2 (NAP-2) for neutrophil stimulating activity. The following functions were tested: cytosolic free calcium changes, chemotaxis in vitro, respiratory burst, exocytosis of azurophil and specific granules from cytochalasin B-pretreated cells, and competition for IL-8 receptor binding. At 1, 10, 100 and 1000 nM, IP-10 was inactive in all assays, in contrast to the reference peptides which exhibited the expected neutrophil stimulating effects. In addition, IP-10 did not induce neutrophil accumulation after intradermal injection in rats, and did not act as IL-8 antagonist.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism
  • Chemokine CXCL1
  • Chemokine CXCL10
  • Chemokines, CXC*
  • Chemotactic Factors / pharmacology
  • Chemotaxis, Leukocyte / drug effects
  • Cytokines / pharmacology*
  • Exocytosis / drug effects
  • Growth Substances / pharmacology
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / pharmacology
  • Neutrophils / drug effects*
  • Respiratory Burst / drug effects


  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokine CXCL10
  • Chemokines, CXC
  • Chemotactic Factors
  • Cytokines
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Calcium