Abstract
We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
MeSH terms
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Animals
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Blotting, Northern / methods
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DNA / genetics
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DNA Primers / chemical synthesis
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DNA Probes / biosynthesis
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DNA, Complementary / genetics
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Drosophila melanogaster / genetics*
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Exons / genetics
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Fluorescent Dyes / metabolism
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Gene Expression Profiling / methods*
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Genes, Insect / genetics
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Genome
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Nucleic Acid Hybridization / methods
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Oligonucleotide Array Sequence Analysis / methods*
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RNA / genetics
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Reproducibility of Results
Substances
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DNA Primers
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DNA Probes
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DNA, Complementary
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Fluorescent Dyes
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RNA
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DNA