Phosphorylation of translation initiation factor eIF2alpha in the brain during pilocarpine-induced status epilepticus in mice

Neurosci Lett. 2004 Mar 11;357(3):191-4. doi: 10.1016/j.neulet.2003.12.093.


In this work, we show extensive phosphorylation of the alpha subunit of translation initiation factor 2 (eIF2alpha) occurring in the brain of mice subjected to 30 min of status epilepticus induced by pilocarpine. eIF2alpha(P) immunoreactivity was detected in the hippocampal pyramidal layer CA1 and CA3, cortex layer V, thalamus and amygdala. After 2 h of recovery, there was a marked decrease in total brain eIF2alpha(P), with the cortex layer V showing the most pronounced loss of anti-eIF2alpha(P) labeling, whereas the CA1 subregion had a significant increase in eIF2alpha(P). These results indicate that inhibition of protein synthesis in experimental models of epilepsy might be due to low levels of eIF2-GTP caused by the phosphorylation of eIF2alpha, and suggest that translational control may contribute to cell fate in the affected areas.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western / methods
  • Brain / anatomy & histology
  • Brain / drug effects
  • Brain / metabolism*
  • Densitometry / methods
  • Disease Models, Animal
  • Immunohistochemistry / methods
  • Male
  • Mice
  • Phosphorylation / drug effects
  • Pilocarpine
  • Status Epilepticus / chemically induced
  • Status Epilepticus / metabolism*
  • Time Factors
  • Transcription Factors / metabolism*


  • Transcription Factors
  • Pilocarpine
  • ELF2 protein, human