Anthocyanin enhances adipocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes

Biochem Biophys Res Commun. 2004 Mar 26;316(1):149-57. doi: 10.1016/j.bbrc.2004.02.031.


Adipocyte dysfunction is strongly associated with the development of obesity and insulin resistance. It is accepted that the regulation of adipocytokine secretion or the adipocyte-specific gene expression is one of the most important targets for the prevention of obesity and amelioration of insulin sensitivity. In this study, we demonstrated that anthocyanins (cyanidin or cyanidin 3-glucoside) have the potency of a unique pharmacological function in isolated rat adipocytes. Treated adipocytes with anthocyanins enhanced adipocytokine (adiponectin and leptin) secretion and up-regulated the adipocyte specific gene expression without activation of PPARgamma in isolated rat adipocytes. The gene expression of adiponectin was also up-regulated in white adipose tissue in mice fed an anthocyanin supplemented diet. As one of the possible mechanisms, AMP-activated protein kinase activation would be associated with these changes, nevertheless, the AMP:ATP ratio was significantly decreased by administration of the anthocyanins. These data suggest that anthocyanins have a potency of unique therapeutic advantage and also have important implications for preventing obesity and diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases
  • Adenine Nucleotides / metabolism
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adiponectin
  • Animals
  • Anthocyanins / chemistry
  • Anthocyanins / pharmacology*
  • Cells, Cultured
  • Cytokines / metabolism
  • Gene Expression / drug effects
  • Glucosides / chemistry
  • Glucosides / pharmacology
  • Intercellular Signaling Peptides and Proteins*
  • Leptin / genetics
  • Leptin / metabolism
  • Ligands
  • Male
  • Multienzyme Complexes / metabolism
  • Protein Serine-Threonine Kinases / metabolism
  • Proteins / genetics
  • Proteins / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Transcription Factors / metabolism


  • Adenine Nucleotides
  • Adiponectin
  • Anthocyanins
  • Cytokines
  • Glucosides
  • Intercellular Signaling Peptides and Proteins
  • Leptin
  • Ligands
  • Multienzyme Complexes
  • Proteins
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors
  • cyanidin-3-O-beta-glucopyranoside
  • cyanidin
  • Protein Serine-Threonine Kinases
  • AMP-Activated Protein Kinases