IGF-I regulates caveolin 1 and IRS1 interaction in caveolae

Biochem Biophys Res Commun. 2004 Mar 26;316(1):240-3. doi: 10.1016/j.bbrc.2004.02.037.


Caveolae are hot spots in IGF-I signalling as suggested by the facts that IGF-I receptors localize in caveolae, directly interact with and tyrosine phosphorylate caveolin 1, the major caveolar protein. Also a number of IGF-IR substrates reside in caveolae, supporting a role of these organelles in the regulation of IGF-I action. Recently, we have demonstrated that IGF-I could specifically regulate Shc phosphorylation in caveolae. Here we show that also IRS1 localizes in this region where it is tyrosine phosphorylated in the presence of IGF-I. Moreover, IRS1 co-immunoprecipitates with caveolin 1 and the specific phosphocaveolin 1-IRS1 interaction is increased by IGF-I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caveolae / drug effects
  • Caveolae / metabolism*
  • Caveolin 1
  • Caveolins / metabolism*
  • Cell Line
  • Insulin Receptor Substrate Proteins
  • Insulin-Like Growth Factor I / pharmacology*
  • Phosphoproteins / chemistry
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Precipitin Tests
  • Tyrosine / metabolism


  • Caveolin 1
  • Caveolins
  • Insulin Receptor Substrate Proteins
  • Phosphoproteins
  • Tyrosine
  • Insulin-Like Growth Factor I