Abstract
A novel compound depudecin inducing the flat phenotype of ras- and src- transformed NIH3T3 cells at a concentration of 1 microgram/ml was isolated from the culture broth of Alternaria brassicicola. Based on its spectroscopic characteristics and X-ray crystallographic analysis of its bis-(1S)-(-)-camphanate, the structure of depudecin was determined to be (2R,3S,4S,5E,7S,8S,9R)-2,9- dihydroxy-3,4;7,8-diepoxy-undeca-5,10-diene.
MeSH terms
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3T3 Cells / drug effects
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Alkadienes / chemistry
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Alkadienes / isolation & purification*
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Alkadienes / pharmacology
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Alternaria / classification
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Alternaria / metabolism*
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Animals
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Cell Transformation, Neoplastic / drug effects*
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Epoxy Compounds / chemistry
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Epoxy Compounds / isolation & purification*
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Epoxy Compounds / pharmacology
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Fatty Alcohols / chemistry
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Fatty Alcohols / isolation & purification*
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Fatty Alcohols / pharmacology
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Fermentation
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Genes, ras
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Genes, src
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Mice
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Phenotype
Substances
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Alkadienes
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Epoxy Compounds
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Fatty Alcohols
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depudecin