Display of Heterologous Antigens on the Bacillus Subtilis Spore Coat Using CotC as a Fusion Partner

Vaccine. 2004 Mar 12;22(9-10):1177-87. doi: 10.1016/j.vaccine.2003.09.031.

Abstract

We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Bacterial / analysis
  • Antibodies, Bacterial / biosynthesis
  • Antigens, Bacterial / chemistry
  • Antigens, Bacterial / immunology*
  • Bacillus subtilis / chemistry
  • Bacillus subtilis / immunology*
  • Bacterial Toxins / immunology
  • Blotting, Western
  • Chromosomes, Bacterial / immunology
  • Cyclohexanones / chemistry
  • Cyclohexanones / immunology*
  • Enterotoxins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli Proteins*
  • Female
  • Genes, Bacterial / genetics
  • Immunity, Mucosal / immunology
  • Immunization
  • Mice
  • Mice, Inbred C57BL
  • Neutralization Tests
  • Peptide Fragments / immunology
  • Recombinant Fusion Proteins / immunology
  • Spores, Bacterial / chemistry
  • Spores, Bacterial / immunology*
  • Tetanus / immunology
  • Tetanus Toxin / immunology
  • Transformation, Bacterial
  • Vaccines, Synthetic / chemistry
  • Vaccines, Synthetic / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Toxins
  • Cyclohexanones
  • Enterotoxins
  • Escherichia coli Proteins
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Tetanus Toxin
  • Vaccines, Synthetic
  • heat-labile enterotoxin, E coli
  • tetanus toxin fragment C
  • 2-crotonyloxymethyl-(4R,5R,6R)-4,5,6-trihydroxycyclohex- 2-enone