Some precautions in using chelators to buffer metals in biological solutions

Cell Calcium. 2004 May;35(5):427-31. doi: 10.1016/j.ceca.2003.10.006.


Chelators and associated computer programs are commonly used to buffer metal ions in biological experiments. This communication discusses common misunderstandings and pitfalls in use of these buffers and provides information on choosing the best metal buffer for different experimental situations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Buffers
  • Chelating Agents / chemistry*
  • Hydrogen-Ion Concentration
  • Metals / chemistry*
  • Models, Chemical*
  • Solutions / chemistry


  • Buffers
  • Chelating Agents
  • Metals
  • Solutions