Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis

J Clin Microbiol. 2004 Mar;42(3):1290-3. doi: 10.1128/JCM.42.3.1290-1293.2004.

Abstract

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Brucella / classification
  • Brucella / genetics
  • Brucella / isolation & purification*
  • Brucella abortus / classification
  • Brucella abortus / genetics
  • Brucella abortus / isolation & purification*
  • Brucella melitensis / classification
  • Brucella melitensis / genetics
  • Brucella melitensis / isolation & purification*
  • Computer Systems
  • DNA Primers
  • Polymerase Chain Reaction / methods*
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics

Substances

  • DNA Primers
  • RNA, Bacterial
  • RNA, Ribosomal, 16S