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, 42 (3), 1316-8

First Isolation of Borrelia Lusitaniae From a Human Patient

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Case Reports

First Isolation of Borrelia Lusitaniae From a Human Patient

M Collares-Pereira et al. J Clin Microbiol.

Abstract

The first human isolate of Borrelia lusitaniae recovered from a Portuguese patient with suspected Lyme borreliosis is described. This isolate, from a chronic skin lesion, is also the first human isolate of Borrelia in Portugal. Different phenotypic and molecular methods are used to characterize it.

Figures

FIG. 1.
FIG. 1.
Sequence identification of the B. lusitaniae human isolate (PoHL1). GenBank accession number, AY209179.
FIG. 2.
FIG. 2.
Midpoint-rooted neighbor-joining tree of partial ospA sequences from the isolate obtained in this study (boldface) and GenBank downloads (*). The Kimura two-parameter model was used to estimate the pairwise distances of the aligned nucleotide sequences (MEGA program, version 2.1). Bootstrap values (500 replicates) are given next to the nodes. The scale indicates the estimated number of nucleotide substitutions per sequence position. GenBank accession numbers: A24014 (ACA1), X14407 (B31), X80257 (PBi), X80256 (PBr), A33374 (PKo), Y10837 (PotiB1), Y10838 (PotiB2), Y10839 (PotiB3), AF227319 (Rio2), AF095941 (UK), and AF369948 (VS219).
FIG. 3.
FIG. 3.
MseI restriction patterns of B. burgdorferi sensu lato rrf-rrl spacer nested-PCR products amplified from cultured strains. Lanes: 1, PoHL1, human isolate; 2, PoTiBG163, tick isolate, B. garinii; 3, PBi, B. garinii; 4 and 5, PKo and PGau, respectively, B. afzelii; 6, PoTiBV6, tick isolate, B. valaisiana; 7 to 9, B31, IP3, and Vs219, respectively, B. burgdorferi sensu stricto; 10, HO14, B. japonica.

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