Diffusion constant in gel electrophoresis at high fields

Electrophoresis. 2004 Mar;25(6):785-9. doi: 10.1002/elps.200305678.

Abstract

We present new measurements of the diffusion constant D in standard (slab-gel) electrophoresis of DNA at fields up to 10 V/cm. Molecules investigated are bacteriophages: T4 of length 173 kbp and lambda of length 48.5 kbp cut by restriction enzyme HindIII. We show, that D increases with the molecule length for electric field E above 5 V/cm. The results are interpreted within the geometration model.

MeSH terms

  • Bacteriophage T4 / genetics
  • DNA / analysis
  • DNA / chemistry*
  • DNA, Viral / chemistry
  • Deoxyribonucleases, Type II Site-Specific / chemistry
  • Diffusion
  • Electrophoresis, Agar Gel*

Substances

  • DNA, Viral
  • DNA
  • Deoxyribonucleases, Type II Site-Specific
  • GTYRAC-specific type II deoxyribonucleases