The differentiation of early embryonic stem cells into adipocytes-like cells

Hum Cell. 2003 Sep;16(3):117-22. doi: 10.1111/j.1749-0774.2003.tb00143.x.

Abstract

An early embryonic stem cell line, EES-6 cells, was established from 2-cell stage embryos of ddY mice. The cells were maintained in an undifferentiated state with D-MEM/F12 medium supplemented with 10% fetal bovine serum (FBS) (GM) and 1 ng of leukemia inhibitory factor (LIF) without any feeder cells. In this study, EES cells were cultured with a medium containing embryotrophic factors (ETFs) which promoted the differentiation of EES cells into white and brown adipocytes-like cells for a period of 5 days. Lipid droplets in brown adipocyte-like cells were stained with Sudan III; however, large lipid-like droplets in white or brown adipocyte-like cells were unstained with either Sudan III or alcian blue. These findings have numerous possibilities for therapeutic use such as regeneration of skin and wound healing.

MeSH terms

  • Adipocytes / cytology*
  • Animals
  • Cell Differentiation* / drug effects
  • Culture Media
  • Embryo, Mammalian / cytology*
  • Interleukin-6 / pharmacology
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Mice
  • Mice, Inbred Strains
  • Receptors, Cytokine / physiology
  • Receptors, OSM-LIF
  • Regenerative Medicine
  • Signal Transduction / drug effects
  • Stem Cells / cytology*

Substances

  • Culture Media
  • Interleukin-6
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lif protein, mouse
  • Lifr protein, mouse
  • Receptors, Cytokine
  • Receptors, OSM-LIF