Identification and distinct regulation of yeast TATA box-containing genes

Cell. 2004 Mar 5;116(5):699-709. doi: 10.1016/s0092-8674(04)00205-3.


Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatin / genetics
  • Chromatin / metabolism
  • Consensus Sequence
  • Gene Expression Regulation, Fungal*
  • Genome, Fungal
  • Humans
  • Phylogeny
  • Saccharomyces / genetics*
  • TATA Box*
  • Transcription Factors, TFII / metabolism
  • Transcription, Genetic


  • Chromatin
  • Transcription Factors, TFII