Microplate and dot immunoassays for the serodiagnosis of tuberculosis

J Lab Clin Med. 1992 Aug;120(2):318-22.


A simple dot enzyme immunoassay based on the recognition of serum IgG antibody to a 30,000 dalton native antigen purified from culture filtrates of Mycobacterium tuberculosis was developed and compared with a standard plate enzyme-linked immunosorbent assay in the serodiagnosis of tuberculosis. The previously described favorable test characteristics of plate enzyme-linked immunoassay were confirmed; although the dot enzyme immunoassay was promising, it was less satisfactory. Dot enzyme immunoassay may have its most promising use as a screening test for situations of limited technical facilities. Both plate enzyme-linked immunoassay and dot enzyme immunoassay had markedly reduced sensitivities in persons with human immunodeficiency virus infection.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Bacterial / immunology
  • Enzyme-Linked Immunosorbent Assay / methods
  • HIV Infections / complications*
  • HIV Seropositivity*
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin G / analysis
  • Immunoglobulin G / blood*
  • Mycobacterium tuberculosis / immunology
  • Prevalence
  • Reference Values
  • Serologic Tests / methods
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / epidemiology
  • Tuberculosis, Pulmonary / etiology


  • Antigens, Bacterial
  • Immunoglobulin G