Expression of glucose transporter isoform GLUT-2 and glucokinase genes in human brain

J Neurochem. 2004 Mar;88(5):1203-10. doi: 10.1046/j.1471-4159.2003.02269.x.


The glucose transporter isoform-2 (GLUT-2) and glucokinase are considered to be components of a glucose sensor system controlling several key processes, and hence may modulate feeding behaviour. We have found GLUT-2 and glucokinase mRNAs in several brain regions, including the ventromedial and arcuate nuclei of the hypothalamus. GLUT-2, glucokinase and glucokinase regulatory protein mRNAs and proteins were present in these areas as determined by biochemical approaches. In addition, glucose-phosphorylating activity with a high apparent Km for glucose that displayed no product inhibition by glucose-6-phosphate was observed. Increased glycaemia after meals may be recognized by specific hypothalamic neurones due to the high Km of GLUT-2 and glucokinase. This enzyme is considered to be the true glucose sensor because it catalyses the rate-limiting step of glucose catabolism its activity being regulated by interaction with glucokinase regulatory protein, that functions as a metabolic sensor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Aged
  • Aged, 80 and over
  • Brain / cytology
  • Brain / metabolism*
  • Brain Chemistry
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics
  • Female
  • Gene Expression*
  • Glucokinase / biosynthesis*
  • Glucokinase / genetics*
  • Glucose / chemistry
  • Glucose / pharmacokinetics
  • Glucose Transporter Type 2
  • Glucose-6-Phosphate / chemistry
  • Humans
  • In Situ Hybridization
  • Male
  • Middle Aged
  • Monosaccharide Transport Proteins / biosynthesis*
  • Monosaccharide Transport Proteins / genetics*
  • Phosphorylation
  • RNA, Messenger / biosynthesis
  • Reference Values
  • Reverse Transcriptase Polymerase Chain Reaction


  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • GCKR protein, human
  • Glucose Transporter Type 2
  • Monosaccharide Transport Proteins
  • RNA, Messenger
  • glucokinase regulatory protein
  • Glucose-6-Phosphate
  • Glucokinase
  • Glucose