Abstract
Trans-arachidonic acids (trans-AA) are products of cis-trans isomerization of arachidonic acid by nitrogen dioxide radical (NO(2)), and occur in vivo, but their metabolism is unknown. We found that hepatic microsomes oxidized trans-AA via cytochrome P450/NADPH system to epoxides, which were hydrolyzed by epoxide hydrolase to diols (DiHETEs). 14,15-trans-AA produced one erythro diol and three threo diols each having one trans double bond.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Arachidonic Acid / chemistry
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Arachidonic Acid / isolation & purification
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Arachidonic Acid / metabolism*
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Cytochrome P-450 Enzyme System / metabolism*
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Epoxy Compounds / chemistry
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Epoxy Compounds / metabolism*
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Microsomes, Liver / enzymology
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Microsomes, Liver / metabolism
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Molecular Structure
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NADP / metabolism*
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Oxidation-Reduction
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Rats
Substances
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Epoxy Compounds
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Arachidonic Acid
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NADP
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Cytochrome P-450 Enzyme System