Immunomodulatory and antitumor activity of Piper longum Linn. and piperine

J Ethnopharmacol. 2004 Feb;90(2-3):339-46. doi: 10.1016/j.jep.2003.10.016.

Abstract

Alcoholic extract of the fruits of the plant Piper longum and its component piperine was studied for their immunomodulatory and antitumor activity. Alcoholic extract of the fruits was 100% toxic at a concentration of 500 microg/ml to Dalton's lymphoma ascites (DLA) cells and 250 microg/ml to Ehrlich ascites carcinoma (EAC) cells. Piperine was found to be cytotoxic towards DLA and EAC cells at a concentration of 250 microg/ml. Alcoholic extract and piperine was also found to produce cytotoxicity towards L929 cells in culture at a concentration of 100 and 50 microg/ml, respectively. Administration of alcoholic extract of Piper longum (10 mg/dose/animal) as well as piperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor to 37.3 and 58.8%, respectively. Administration of Piper longum extract and piperine increased the total WBC count to 142.8 and 138.9%, respectively, in Balb/c mice. The number of plaque forming cells also enhanced significantly by the administration of the extract (100.3%) and piperine (71.4%) on 5th day after immunization. Bone marrow cellularity and alpha-esterase positive cells were also increased by the administration of Piper longum extract and piperine.

Publication types

  • Comparative Study

MeSH terms

  • Adjuvants, Immunologic / isolation & purification
  • Adjuvants, Immunologic / pharmacology
  • Adjuvants, Immunologic / therapeutic use*
  • Alkaloids*
  • Animals
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antineoplastic Agents, Phytogenic / therapeutic use*
  • Benzodioxoles
  • Carcinoma, Ehrlich Tumor / drug therapy*
  • Carcinoma, Ehrlich Tumor / immunology
  • Mice
  • Mice, Inbred BALB C
  • Piper*
  • Piperidines / isolation & purification
  • Piperidines / pharmacology
  • Piperidines / therapeutic use*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Polyunsaturated Alkamides
  • Sheep
  • Xenograft Model Antitumor Assays / methods

Substances

  • Adjuvants, Immunologic
  • Alkaloids
  • Antineoplastic Agents, Phytogenic
  • Benzodioxoles
  • Piperidines
  • Plant Extracts
  • Polyunsaturated Alkamides
  • piperine