Evaluation of anesthesia effects on [18F]FDG uptake in mouse brain and heart using small animal PET

Nucl Med Biol. 2004 Feb;31(2):251-6. doi: 10.1016/S0969-8051(03)00124-0.


This study evaluates effects of anesthesia on (18)F-FDG (FDG) uptake in mouse brain and heart to establish the basic conditions of small animal PET imaging. Prior to FDG injection, 12 mice were anesthetized with isoflurane gas; 11 mice were anesthetized with an intraperitoneal injection of a ketamine/xylazine mixture; and 11 mice were awake. In isoflurane and ketamine/xylazine conditions, FDG brain uptake (%ID/g) was significantly lower than in controls. Conversely, in the isoflurane condition, %ID/g in heart was significantly higher than in controls, whereas heart uptake in ketamine/xylazine mice was significantly lower. Results suggest that anesthesia impedes FDG uptake in mouse brain and affects FDG uptake in heart; however, the effects in the brain and heart differ depending on the type of anesthesia used.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Anesthesia / methods*
  • Anesthetics / pharmacology*
  • Animals
  • Brain / diagnostic imaging
  • Brain / drug effects
  • Brain / metabolism*
  • Fluorodeoxyglucose F18 / pharmacokinetics*
  • Heart / diagnostic imaging
  • Heart / drug effects
  • Isoflurane / pharmacology
  • Ketamine / pharmacology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Myocardium / metabolism*
  • Positron-Emission Tomography / methods*
  • Radiopharmaceuticals / pharmacokinetics
  • Xylazine / pharmacology


  • Anesthetics
  • Radiopharmaceuticals
  • Fluorodeoxyglucose F18
  • Xylazine
  • Ketamine
  • Isoflurane