Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development

Brain Res Dev Brain Res. 2004 Mar 22;149(1):1-8. doi: 10.1016/j.devbrainres.2003.10.011.


During early postnatal development, a switch occurs between eEF1A-1/EF-1alpha and eEF1A-2/S1, homologous peptide elongation factors, in brain, heart, and skeletal muscle; eEF1A-2/S1 becomes the major form expressed in maturity. By immunofluorescent labeling, we detected both homologues in the developing brains of wild-type and wasted mutant mice, carrying a deletion in the eEF1A-2/S1 gene; we found that brain expression of eEF1A-2/S1 protein is restricted to mature, terminally differentiated neurons, and coincides with the disappearance of eEF1A-1/EF-1alpha 20 days after birth. Furthermore, no elongation factor 1A is present in wasted mutant mice neurons following the developmental switch, indicating that the genetic regulation silencing eEF1A-1/EF-1alpha is still functional.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Central Nervous System / cytology
  • Central Nervous System / growth & development*
  • Central Nervous System / metabolism*
  • Embryo, Mammalian
  • Fluorescent Antibody Technique / methods
  • Gene Expression Regulation, Developmental
  • Glial Fibrillary Acidic Protein / metabolism
  • Heterozygote
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Microscopy, Confocal / methods
  • Mutation
  • Neurofilament Proteins / metabolism
  • Neurons / metabolism
  • Peptide Elongation Factor 1 / genetics
  • Peptide Elongation Factor 1 / metabolism*
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction / methods


  • Eef1a2 protein, mouse
  • Glial Fibrillary Acidic Protein
  • Neurofilament Proteins
  • Peptide Elongation Factor 1
  • RNA, Messenger
  • neurofilament protein NF 68