SHN-1, a Shank homologue in C. elegans, affects defecation rhythm via the inositol-1,4,5-trisphosphate receptor

FEBS Lett. 2004 Mar 12;561(1-3):29-36. doi: 10.1016/S0014-5793(04)00107-3.


Protein localization in the postsynaptic density (PSD) of neurons is mediated by scaffolding proteins such as PSD-95 and Shank, which ensure proper function of receptors at the membrane. The Shank family of scaffolding proteins contain PDZ (PSD-95, Dlg, and ZO-1) domains and have been implicated in the localizations of many receptor proteins including glutamate receptors in mammals. We have identified and characterized shn-1, the only homologue of Shank in Caenorhabditis elegans. The shn-1 gene shows approximately 40% identity over 1000 amino acids to rat Shanks. SHN-1 protein is localized in various tissues including neurons, pharynx and intestine. RNAi suppression of SHN-1 did not cause lethality or developmental abnormality. However, suppression of SHN-1 in the itr-1 (sa73) mutant, which has a defective inositol-1,4,5-trisphosphate (IP(3)) receptor, resulted in animals with altered defecation rhythm. Our data suggest a possible role of SHN-1 in affecting function of IP(3) receptors in C. elegans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / physiology
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Calcium Channels / genetics*
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology*
  • Defecation / genetics*
  • Inositol 1,4,5-Trisphosphate Receptors
  • Molecular Sequence Data
  • Mutation
  • Nerve Tissue Proteins
  • Periodicity
  • RNA, Small Interfering / pharmacology
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Sequence Homology
  • Tissue Distribution


  • Adaptor Proteins, Signal Transducing
  • Caenorhabditis elegans Proteins
  • Calcium Channels
  • Carrier Proteins
  • Inositol 1,4,5-Trisphosphate Receptors
  • Nerve Tissue Proteins
  • RNA, Small Interfering
  • Receptors, Cytoplasmic and Nuclear
  • SHN-1 protein, C elegans
  • Shank1 protein, rat