Functional analysis of PU.1 domains in monocyte-specific gene regulation

FEBS Lett. 2004 Mar 12;561(1-3):63-8. doi: 10.1016/S0014-5793(04)00116-4.


The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Cell Division
  • Cell Size
  • Gene Expression Regulation*
  • Genetic Vectors
  • Immunophenotyping
  • Interleukin-6 / biosynthesis
  • Lipopolysaccharides / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Monocytes / metabolism*
  • Mutation
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / chemistry
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*
  • Sequence Deletion
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / physiology*
  • Transfection


  • Interleukin-6
  • Lipopolysaccharides
  • Proto-Oncogene Proteins
  • Trans-Activators
  • proto-oncogene protein Spi-1