Alpha-chimaerin exists in a functional complex with the Cdk5 kinase in brain

FEBS Lett. 2004 Mar 12;561(1-3):177-80. doi: 10.1016/S0014-5793(04)00174-7.


Cyclin-dependent kinase 5 (Cdk5) in association with its neuronal activators p35 and p39 shows a complex involvement in the control of neurocytoskeletal dynamics. Here we show that alpha-chimaerin, a GTPase-activating protein specific for Rac and Cdc42, is a p35-binding protein. The interaction domains of p35 and alpha-chimaerin were delineated. In transfected HeLa cells, p35 and alpha-chimaerin displayed an overlapping distribution pattern and they could be co-immunoprecipitated from the cell lysate. As alpha-chimaerin has a regulatory function in actin repolymerization, these results suggested that the regulation of neurocytoskeleton dynamics by Cdk5 is mediated at least in part via alpha-chimaerin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / enzymology
  • Chimerin 1 / analysis
  • Chimerin 1 / genetics
  • Chimerin 1 / metabolism*
  • Cyclin-Dependent Kinase 5
  • Cyclin-Dependent Kinases / analysis
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / metabolism*
  • Cytoskeleton / metabolism
  • Gene Library
  • HeLa Cells
  • Humans
  • Neurons
  • Precipitin Tests
  • Protein Binding
  • Transfection
  • Two-Hybrid System Techniques


  • Chimerin 1
  • Cyclin-Dependent Kinase 5
  • CDK5 protein, human
  • Cyclin-Dependent Kinases