Verification of components of acellular pertussis vaccines that have been distributed solely, been in routine use for the last two decades and contributed greatly to control of pertussis in Japan

Biologicals. 2004 Mar;32(1):29-35. doi: 10.1016/j.biologicals.2003.11.001.


An ideal acellular pertussis vaccine is now under investigation worldwide. We have had acellular pertussis vaccines available for the last 22 years, which contributed greatly to the control of pertussis in Japan, although it has not been known whether they are one of ideal acellular pertussis vaccines or not. Moreover, the formulations of acellular pertussis vaccines that we have been using have not been widely recognized. Serum samples were taken from recipients of the T type, B type, and two-component acellular pertussis vaccine and assayed by ELISA for anti-PT, anti-FHA, and anti-69 kD OMP antibody levels and by the agglutination test. Although it was shown that T type vaccine contained four components (PT, FHA, 69 kD OMP, agglutingen), B type vaccine contained three components (PT, FHA, 69 kD OMP) and the two-component vaccine contained PT and FHA, it was concluded that PT and FHA were essential and common antigens contained in all three acellular pertussis vaccines in Japan. The national monitoring system for adverse effects of routine immunization demonstrated low reactogenicity of DTaP in Japan. This resulted in high acceptance rates of DTaP and in virtual control of pertussis.

MeSH terms

  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fever
  • Humans
  • Infant
  • Japan
  • Male
  • Pertussis Vaccine / chemistry*
  • Prospective Studies
  • Time Factors
  • Vaccines
  • Vaccines, Acellular / chemistry*
  • Virulence Factors, Bordetella / immunology*
  • Whooping Cough / epidemiology
  • Whooping Cough / prevention & control*


  • Pertussis Vaccine
  • Vaccines
  • Vaccines, Acellular
  • Virulence Factors, Bordetella