The biosynthesis of anticoagulant heparan sulfate by the heparan sulfate 3-O-sulfotransferase isoform 5

Biochim Biophys Acta. 2004 Mar 17;1671(1-3):34-43. doi: 10.1016/j.bbagen.2003.12.010.


The role of heparan sulfate (HS) in regulating blood coagulation has a wide range of clinical implications. In this study, we investigated the role of 3-O-sulfotransferase isoform 5 (3-OST-5) in generating anticoagulant HS in vivo. A Chinese hamster ovary cell line (3OST5/CHO) stably expressing 3-OST-5 was generated. The expression of 3-OST-5 in 3OST5/CHO cells was confirmed by Northern blot analysis, RT-PCR, and the disaccharide analyses of the HS from the cells. We also determined the effects of the HS from 3OST5/CHO on antithrombin-mediated inhibition of factor Xa. Fluorescently labeled antithrombin bound to the surface of 3OST5/CHO cells, suggesting that the antithrombin-binding HS is indeed present on the cell surface. Our results demonstrate that the 3-OST-5 gene is capable of synthesizing anticoagulant HS in CHO cells and has the potential to contribute to the biosynthesis of anticoagulant HS in humans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anticoagulants / chemistry
  • Anticoagulants / metabolism*
  • Antithrombins / metabolism
  • CHO Cells
  • Cricetinae
  • Disaccharides / analysis
  • Electrophoresis
  • Factor Xa / metabolism
  • Heparitin Sulfate / biosynthesis*
  • Heparitin Sulfate / chemistry
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Protein Binding
  • Sulfotransferases / genetics
  • Sulfotransferases / metabolism*


  • Anticoagulants
  • Antithrombins
  • Disaccharides
  • Isoenzymes
  • Heparitin Sulfate
  • Sulfotransferases
  • heparitin sulfotransferase
  • Factor Xa