The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function

Curr Biol. 2004 Mar 9;14(5):412-7. doi: 10.1016/j.cub.2004.02.032.

Abstract

Directional cell expansion in interphase and nuclear and cell division in M-phase are mediated by four microtubule arrays, three of which are unique to plants: the interphase array, the preprophase band, and the phragmoplast. The plant microtubule-associated protein MAP65 has been identified as a key structural component in these arrays. The Arabidopsis genome has nine MAP65 genes, and here we show that one, AtMAP65-3/PLE, locates only to the mitotic arrays and is essential for cytokinesis. The Arabidopsis pleiade (ple) alleles are single recessive mutations, and we show that these mutations are in the AtMAP65-3 gene. Moreover, these mutations cause C-terminal truncations that abolish microtubule binding. In the ple mutants the anaphase spindle is normal, and the cytokinetic phragmoplast can form but is distorted; not only is it wider, but the midline, the region where oppositely oriented microtubules overlap, is unusually expanded. Here we present data that demonstrate an essential role for AtMAP65-3/PLE in cytokinesis in plant cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Arabidopsis
  • Cell Division / physiology
  • Centrifugation
  • DNA Primers
  • DNA, Complementary / genetics
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Luminescent Proteins / metabolism
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Microtubules / physiology*
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism*
  • Recombinant Proteins
  • Sequence Analysis, DNA
  • Spindle Apparatus / metabolism
  • Spindle Apparatus / physiology*

Substances

  • DNA Primers
  • DNA, Complementary
  • Luminescent Proteins
  • MAP4
  • Microtubule-Associated Proteins
  • Plant Proteins
  • Recombinant Proteins
  • Green Fluorescent Proteins