Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment

Graefes Arch Clin Exp Ophthalmol. 2004 May;242(5):437-41. doi: 10.1007/s00417-004-0861-7. Epub 2004 Mar 17.


Purpose: To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment.

Methods: Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody.

Results: The p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment.

Conclusion: These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.

MeSH terms

  • Animals
  • Cell Cycle Proteins / metabolism*
  • Cyclin D1 / metabolism*
  • Cyclin-Dependent Kinase Inhibitor p27
  • Fluorescent Antibody Technique, Indirect
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • Pigment Epithelium of Eye / metabolism
  • Proliferating Cell Nuclear Antigen / metabolism*
  • Retina / metabolism
  • Retinal Detachment / metabolism*
  • Tumor Suppressor Proteins / metabolism*


  • Cdkn1b protein, mouse
  • Cell Cycle Proteins
  • Proliferating Cell Nuclear Antigen
  • Tumor Suppressor Proteins
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor p27