Abstract
During convergent extension in Drosophila, polarized cell movements cause the germband to narrow along the dorsal-ventral (D-V) axis and more than double in length along the anterior-posterior (A-P) axis. This tissue remodeling requires the correct patterning of gene expression along the A-P axis, perpendicular to the direction of cell movement. Here, we demonstrate that A-P patterning information results in the polarized localization of cortical proteins in intercalating cells. In particular, cell fate differences conferred by striped expression of the even-skipped and runt pair-rule genes are both necessary and sufficient to orient planar polarity. This polarity consists of an enrichment of nonmuscle myosin II at A-P cell borders and Bazooka/PAR-3 protein at the reciprocal D-V cell borders. Moreover, bazooka mutants are defective for germband extension. These results indicate that spatial patterns of gene expression coordinate planar polarity across a multicellular population through the localized distribution of proteins required for cell movement.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Antigens, CD
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Armadillo Domain Proteins
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Body Patterning / genetics
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Body Patterning / physiology*
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Carrier Proteins / metabolism
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Cell Adhesion Molecules / metabolism
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Cell Movement
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Cell Polarity / genetics
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Cell Polarity / physiology*
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DNA-Binding Proteins / metabolism
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Drosophila / physiology*
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Drosophila Proteins / metabolism
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Embryo, Nonmammalian
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Embryonic Induction
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Female
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Gene Expression Regulation / physiology*
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Genes, Insect / genetics
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Glycoproteins / physiology*
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Homeodomain Proteins / metabolism
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Immunoglobulins / physiology*
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Immunohistochemistry / methods
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Intracellular Signaling Peptides and Proteins*
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Male
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Mutation
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Myosin Type II / metabolism
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Nuclear Proteins
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Receptors, Cell Surface
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Signaling Lymphocytic Activation Molecule Family Member 1
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Trans-Activators / metabolism
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Transcription Factors / metabolism
Substances
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ARM protein, Drosophila
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Antigens, CD
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Armadillo Domain Proteins
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Carrier Proteins
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Cell Adhesion Molecules
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DNA-Binding Proteins
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Drosophila Proteins
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Glycoproteins
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Homeodomain Proteins
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Immunoglobulins
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Intracellular Signaling Peptides and Proteins
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Nuclear Proteins
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Receptors, Cell Surface
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Trans-Activators
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Transcription Factors
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baz protein, Drosophila
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eve protein, Drosophila
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run protein, Drosophila
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Signaling Lymphocytic Activation Molecule Family Member 1
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Myosin Type II