Bone marrow stromal cells produce nerve growth factor and glial cell line-derived neurotrophic factors

Biochem Biophys Res Commun. 2004 Apr 9;316(3):753-4. doi: 10.1016/j.bbrc.2004.02.111.


Bone marrow stromal cells (BMSC) have attracted interest through their possible use for cell therapy in neurological diseases. Recent reports demonstrated that these cells are able to migrate and have potential for neuronal differentiation after transplantation into brain parenchyma. The objective of this work was determine whether rat BMSC express NGF and GDNF, in order to study its potential application for treatment of neurodegenerative diseases. BMSC were harvested from male rats and cultured in DMEM supplemented with 20% fetal bovine serum. At passage 6 the total RNA was isolated using TriZol reactive. RT-PCRs to evaluate the expression of NGF and GDNF using specific primers were carried out. Our results indicate that rat BMSC have potential to produce NGF and GDNF. We have not found any report in favor of GDNF or NGF production from rat BMSC.

MeSH terms

  • Animals
  • Bone Marrow Cells / metabolism*
  • Cells, Cultured
  • Glial Cell Line-Derived Neurotrophic Factor
  • Guanidines / pharmacology
  • Male
  • Nerve Growth Factor / biosynthesis*
  • Nerve Growth Factors / biosynthesis*
  • Phenols / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction
  • Solutions / pharmacology
  • Stromal Cells / metabolism*
  • Temperature
  • Time Factors


  • Gdnf protein, rat
  • Glial Cell Line-Derived Neurotrophic Factor
  • Guanidines
  • Nerve Growth Factors
  • Phenols
  • Solutions
  • trizol
  • Nerve Growth Factor