Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci

Antimicrob Agents Chemother. 1992 Apr;36(4):867-9. doi: 10.1128/AAC.36.4.867.

Abstract

The VanA ligase encoded by the vancomycin resistance plasmid pIP816 of Enterococcus faecium BM4147 condenses D-alanine with various D-2-hydroxy and D-2-amino acids in vitro. D-Lactate added to the culture medium restored the vancomycin resistance of a strain that does not produce the VanH dehydrogenase and therefore appears to be a substrate of VanA in vivo.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Drug Resistance, Microbial
  • Enterococcus faecium / drug effects
  • Enterococcus faecium / metabolism*
  • Lactates / metabolism*
  • Lactic Acid
  • Microbial Sensitivity Tests
  • Peptidoglycan / metabolism*
  • Vancomycin / metabolism
  • Vancomycin / pharmacology

Substances

  • Lactates
  • Peptidoglycan
  • Lactic Acid
  • Vancomycin