A quick and inexpensive method for removing polysaccharides from plant genomic DNA

Biotechniques. 1992 Jul;13(1):52-4, 56.

Abstract

A quick and inexpensive method has been demonstrated to remove polysaccharide contamination from plant DNA. Isolated plant genomic DNA with polysaccharide contaminants was dissolved in TE (10 mM Tris-HCl, pH 7.4, 1 mM EDTA) with NaCl ranging from 0.5-3.0 M, then precipitated with two volumes of ethanol. Most of the polysaccharides were removed effectively in a single high-salt precipitation at 1.0-2.5 M NaCl. At 3.0 M NaCl, the salt precipitated out of solution. Purified DNA was easily digested by either HindIII or EcoRI and was satisfactory as a template for PCR. The results show that high-salt precipitation effectively removed polysaccharides and their inhibitory effects on restriction enzyme and Taq polymerase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / isolation & purification*
  • Ethanol
  • Genetic Techniques / economics
  • Plants / genetics*
  • Polysaccharides / isolation & purification*
  • Time Factors

Substances

  • Polysaccharides
  • Ethanol
  • DNA