cAMP detection methods in HTS: selecting the best from the rest

Nat Rev Drug Discov. 2004 Feb;3(2):125-35. doi: 10.1038/nrd1306.


The number of technologies that enable high-throughput functional screening of G-protein-coupled receptors has expanded markedly over the past 5 years. Consequently, choosing the most appropriate technology can be a daunting task, particularly for Gi- or Gs-coupled receptors. The most common systems for cyclic AMP detection are reviewed, highlighting the practical and theoretical aspects that are important in their application to high-throughput screening. Current technologies can do the job, but it is likely that the future may require development of technologies that provide even greater biological information.

Publication types

  • Review

MeSH terms

  • Animals
  • Cyclic AMP / analysis*
  • Cyclic AMP / biosynthesis
  • Cyclic AMP / genetics
  • Drug Design
  • Drug Evaluation, Preclinical / methods*
  • Genes, Reporter
  • Humans


  • Cyclic AMP