Human hepatic cell uptake of resveratrol: involvement of both passive diffusion and carrier-mediated process

Biochem Biophys Res Commun. 2004 Apr 16;316(4):1132-7. doi: 10.1016/j.bbrc.2004.02.164.


This work reports significant advances on the transport in hepatic cells of resveratrol, a natural polyphenol with potent protective properties. First, we describe a new simple technique to qualitatively follow resveratrol cell uptake and intracellular distribution, based on resveratrol fluorescent properties. Second, the time-course study and the quantification of (3)H-labelled resveratrol uptake have been performed using human hepatic derived cells (HepG2 tumor cells) and hepatocytes. The temperature-dependence of the kinetics of uptake as well as the cis-inhibition experiments agree with the involvement of a carrier-mediated transport in addition to passive diffusion. The decrease of passive uptake resulting from resveratrol binding to serum proteins brings to light a mediated mechanism in physiological situation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Availability
  • Biological Transport, Active
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Line, Tumor
  • Diffusion
  • Hepatoblastoma / metabolism*
  • Hepatocytes / metabolism*
  • Humans
  • Metabolic Clearance Rate
  • Resveratrol
  • Stilbenes / pharmacokinetics*
  • Temperature
  • Tissue Distribution


  • Carrier Proteins
  • Stilbenes
  • Resveratrol