N-Dansyl-N'-ethylthiourea for the fluorometric detection of heavy metal ions

M Schuster; M Sandor

doi:10.1007/s0021663560326

N-Dansyl-N'-ethylthiourea for the fluorometric detection of heavy metal ions

Anal Bioanal Chem. 1996 Oct;356(5):326-30. doi: 10.1007/s0021663560326.

Authors

M Schuster¹, M Sandor

Affiliation

¹ Anorganisch-chemisches Institut der Technischen Universität München, Lichtenbergstrasse 4, D-85747, Garching, Germany.

PMID: 15045233
DOI: 10.1007/s0021663560326

Abstract

N-Dansyl-N'-ethylthiourea (DET) forms fluorescent chelates with a large number of heavy metal ions. The influence of the pH-value on the luminescence of DET and its metal chelates was investigated. The addition of Cu(II) to DET causes a bathochromic shift of the emission maximum, which is linearly dependent on the Cu(II) concentration. Low detection limits and a wide linear range of detection are achieved by HPLC using RP 18 columns as stationary phase. Also presented are first measurements of fluorescence decay times of the ligand as well as some complexes.