Anti-tumor activity of chemokine is affected by both kinds of tumors and the activation state of the host's immune system: implications for chemokine-based cancer immunotherapy

Biochem Biophys Res Commun. 2004 Apr 23;317(1):68-76. doi: 10.1016/j.bbrc.2004.03.013.


In this study, we screened the anti-tumor activity of murine chemokines including CCL17, CCL19, CCL20, CCL21, CCL22, CCL27, XCL1, and CX3CL1 by inoculating murine B16BL6, CT26, or OV-HM tumor cells, all of which were transfected with chemokine-expressing fiber-mutant adenovirus vector, into immunocompetent mice. A tumor-suppressive effect was observed in mice inoculated with CCL19/B16BL6 and XCL1/B16BL6, and CCL22/OV-HM showed considerable retardation in tumor growth. In the cured mice inoculated with CCL22/OV-HM, a long-term specific immune protection against parental tumor was developed. However, we were unable to identify the chemokine that had a suppressive activity common to all three tumor models. Furthermore, an experiment using chemokine-transfected B16BL6 cells was also performed on mice sensitized with melanoma-associated antigen. A drastic enhancement of the frequency of complete rejection was observed in mice inoculated with CCL17-, CCL19-, CCL22-, and CCL27-transfected B16BL6. Altogether, our results suggest that the tumor-suppressive activity of chemokine-gene immunotherapy is greatly influenced by the kind of tumor and the activation state of the host's immune system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Cell Line, Tumor
  • Chemokines / biosynthesis
  • Chemokines / genetics*
  • Chemokines / immunology*
  • Chemotaxis
  • Gene Expression
  • Genetic Therapy / methods
  • Genetic Vectors / genetics
  • Humans
  • Immunotherapy / methods*
  • Membrane Glycoproteins / immunology
  • Mice
  • Neoplasm Proteins / immunology
  • Neoplasms / genetics
  • Neoplasms / immunology*
  • Neoplasms / therapy
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Transduction, Genetic
  • Transfection
  • gp100 Melanoma Antigen


  • Chemokines
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • PMEL protein, human
  • Pmel protein, mouse
  • RNA, Messenger
  • Recombinant Proteins
  • gp100 Melanoma Antigen