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. 2004 Apr;57(4):360-4.
doi: 10.1136/jcp.2003.012369.

Unfavourable Prognosis of Patients With Trisomy 18q21 Detected by Fluorescence in Situ Hybridisation in t(11;18) Negative, Surgically Resected, Gastrointestinal B Cell Lymphomas

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Free PMC article

Unfavourable Prognosis of Patients With Trisomy 18q21 Detected by Fluorescence in Situ Hybridisation in t(11;18) Negative, Surgically Resected, Gastrointestinal B Cell Lymphomas

J Krugmann et al. J Clin Pathol. .
Free PMC article

Abstract

Background: The most frequent cytogenetic alteration in gastrointestinal (GI) B cell lymphoma (BCL) is t(11;18)(q21;q21). GI B cell non-Hodgkin lymphomas lacking this translocation vary in their biology and clinical outcome. The t(11;18) negative subgroup shows increased numerical changes of chromosome 18, although its clinical relevance remains unknown.

Methods: Thirty surgically resected primary GI BCLs were examined-11 low grade marginal zone mucosa associated lymphoid tissue (MALT) lymphomas, four marginal zone lymphomas with diffuse large BCL (DLBCL), and 15 de novo DLBCLs. Chromosome 18 aberrations were examined using interphase fluorescence in situ hybridisation. Trisomy 18 was studied applying a centromere 18 probe and a dual colour probe for the MALT1 gene at 18q21.

Results: Using the MALT1 probe, only one marginal zone MALT lymphoma had a break apart pattern, indicating t(11;18) or variants. In the GI BCLs lacking MALT1 breaks, trisomy 18q21 was seen in seven patients (four with complete trisomy 18 and three with partial trisomy of 18q21). Trisomy 18q21 was found in two of 10 low grade MALT lymphomas and five of 19 GI BCLs with large cell component. Six of 17 patients with trisomy 18q21 presented with >/= stage II and one of 12 with stage I disease. Trisomy 18q21 was associated with significantly shorter disease specific survival in the whole group and GI BCLs with large cell component, but not in the low grade group.

Conclusions: Trisomy 18q21, including MALT1, may be associated with advanced tumour stage and may be a predictor of poor outcome in surgically resected primary GI BCLs.

Figures

Figure 1
Figure 1
Detection of partial trisomy 18q21 in cell nuclei isolated from one case with low grade marginal zone lymphoma of the mucosa associated lymphoid tissue (MALT) type by dual colour fluorescence in situ hybridisation (FISH) using MALT1 flanking probes. In red, phage 1 artificial chromosomes (PACs) 83A16 and 119K19 proximal to MALT1; in green, PACs 628B12 and 124N11 distal to MALT1. Several DAPI stained nuclei (blue) with three copies of MALT1 (mixed red/green or yellow signal) are shown, whereas FISH with the centromere 18 probe on nuclei from this same case showed normal disomy (not shown).
Figure 2
Figure 2
(A) Kaplan–Meier curve of disease specific survival of all patients with gastrointestinal (GI) B cell lymphoma with regard to MALT1 status (p  =  0.0458). (B) Kaplan–Meier curve of disease specific survival of patients with GI B cell lymphoma and a large cell lymphoma component with regard to MALT1 status (p  =  0.0447).

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