Glycine prevents hepatic fibrosis by preventing the accumulation of collagen in rats with alcoholic liver injury

Pol J Pharmacol. Jan-Feb 2004;56(1):121-8.

Abstract

We studied the effect of administering glycine, a non-essential amino acid, on liver collagen content and its characteristics in experimental hepatotoxic Wistar rats. All the rats were fed standard pellet diet. Hepatotoxicity was induced by orally administering ethanol (7.9 g kg(-1)) for 30 days. Control rats were given isocaloric glucose solution. Glycine was administered subsequently at a dose of 0.6 g kg(-1) po every day, along with alcohol for the next 30 days. Alcohol administration significantly elevated the levels of liver hydroxyproline and total collagen content, cross-linked fluorescence, shrinkage temperature and lipid peroxidation, whereas it significantly decreased the solubility of liver collagen as compared with the control rats. Simultaneous glycine supplementation to alcohol-fed rats significantly reduced the levels of liver hydroxyproline and total collagen content, cross-linked fluorescence, shrinkage temperature and lipid peroxidation and enhanced the solubility of liver collagen as compared with the unsupplemented alcohol-fed rats. In conclusion, administration of glycine had a positive influence both on the quantitative and qualitative properties of hepatic collagen in alcoholic liver injury.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Oral
  • Animals
  • Collagen / adverse effects
  • Collagen / drug effects*
  • Collagen / metabolism*
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Ethanol / administration & dosage
  • Ethanol / adverse effects
  • Glucose / administration & dosage
  • Glucose / metabolism
  • Glycine / administration & dosage
  • Glycine / physiology
  • Glycine / therapeutic use*
  • Hydroxyproline / chemistry
  • Hydroxyproline / drug effects
  • Hydroxyproline / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / chemistry
  • Liver / drug effects
  • Liver / metabolism
  • Liver Cirrhosis, Alcoholic / drug therapy
  • Liver Cirrhosis, Alcoholic / etiology
  • Liver Cirrhosis, Alcoholic / prevention & control*
  • Liver Regeneration / physiology*
  • Male
  • Rats
  • Rats, Wistar
  • Spectrometry, Fluorescence
  • Temperature
  • Thiobarbituric Acid Reactive Substances / chemistry
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Time Factors

Substances

  • Thiobarbituric Acid Reactive Substances
  • Ethanol
  • Collagen
  • Glucose
  • Hydroxyproline
  • Glycine