The murine inhibitory receptor mSiglec-E is expressed broadly on cells of the innate immune system whereas mSiglec-F is restricted to eosinophils

Eur J Immunol. 2004 Apr;34(4):1175-84. doi: 10.1002/eji.200324723.


Murine (m) Siglec-E and mSiglec-F are recently discovered murine sialic acid-binding Ig-like lectins with tyrosine-based inhibitory signaling motifs. They are postulated to be the orthologs of human (h) siglec-7, -8 or -9 and siglec-5, respectively. We report here the first detailed characterization of mSiglec-E, and compare its expression pattern with mSiglec-F. Similar to hSiglec-7, mSiglec-E preferred alpha 2-8-linked disialic acid over alpha 2-3- and alpha 2-6-linked sialic acids. Using a specific Ab, FACS analysis demonstrated that mSiglec-E was expressed mainly on neutrophils in blood and their immature precursors in bone marrow. mSiglec-E was present on peritoneal cavity macrophages and on subsets of mature NK cells and splenic dendritic cells. mSiglec-E was also found ona novel population of peritoneal cavity B-1a-like cells and a subset of splenic B cells enriched in transitional T2 and marginal zone B cells. In striking contrast to mSiglec-E, mSiglec-F was expressed predominantly on eosinophils in blood and their precursors in the bone marrow. The distinct and largely non-overlapping expression profiles of mSiglec-E and mSiglec-F suggest that they play non-redundant roles in the innate immune system. mSiglec-E is likely to modulate the functions of several types of effector cells, whereas mSiglec-F is likely to be more restricted to eosinophil biology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / immunology*
  • Antigens, CD / metabolism
  • Antigens, Differentiation, B-Lymphocyte / immunology*
  • Antigens, Differentiation, B-Lymphocyte / metabolism
  • Antigens, Differentiation, Myelomonocytic / immunology*
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Bone Marrow Cells / immunology
  • Bone Marrow Cells / metabolism
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Eosinophils / immunology*
  • Eosinophils / metabolism
  • Flow Cytometry
  • Humans
  • Immunity, Innate*
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Macrophages / immunology
  • Macrophages / metabolism
  • Mice
  • Molecular Sequence Data
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • Peritoneal Cavity / cytology
  • Sequence Homology, Nucleic Acid
  • Sialic Acid Binding Immunoglobulin-like Lectins
  • Spleen / cytology
  • Spleen / immunology
  • Stem Cells / immunology
  • Stem Cells / metabolism


  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, Myelomonocytic
  • Sialic Acid Binding Immunoglobulin-like Lectins
  • Siglece protein, mouse
  • Siglecf protein, mouse