Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

Can J Microbiol. 2001 Jan;47(1):77-80. doi: 10.1139/w00-128.


Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ.Tag Amino Acid Analysis Method was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids, Cyclic / analysis*
  • Amino Acids, Cyclic / metabolism
  • Bacteria / enzymology*
  • Brassica napus
  • Brassica rapa / chemistry
  • Brassica rapa / growth & development
  • Brassica rapa / microbiology*
  • Carbon-Carbon Lyases / metabolism*
  • Ethylenes / metabolism
  • Plant Roots / chemistry
  • Plant Roots / growth & development*
  • Plant Roots / microbiology*
  • Seedlings / chemistry
  • Seedlings / growth & development
  • Seedlings / microbiology


  • Amino Acids, Cyclic
  • Ethylenes
  • 1-aminocyclopropane-1-carboxylic acid
  • ethylene
  • 1-aminocyclopropane-1-carboxylate deaminase
  • Carbon-Carbon Lyases