Abstract
A gene encoding high-level resistance to ethidium bromide (EB) and antiseptics was isolated from a transferable plasmid, pTZ22, in Staphylococcus aureus. DNA sequencing revealed that the plasmid has two copies of the ebr gene that normally mediates low-level resistance to EB and antiseptics. The efflux rate for EB of strains with duplicated ebr genes was twice the rate of strains with a single ebr gene. It was concluded that the duplication of ebr is responsible for the high-level resistance to EB and antiseptics.
MeSH terms
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Amino Acid Sequence
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Anti-Infective Agents, Local / pharmacology*
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Base Sequence
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Chromosome Deletion
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Chromosomes, Bacterial
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Drug Resistance, Microbial
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Ethidium / metabolism
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Ethidium / pharmacology*
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Molecular Sequence Data
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Plasmids / genetics
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / genetics*
Substances
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Anti-Infective Agents, Local
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Ethidium
Associated data
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GENBANK/S39996
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GENBANK/S40001
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GENBANK/S40008
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GENBANK/S40009
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GENBANK/S40010
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GENBANK/X62587
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GENBANK/X68838
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GENBANK/X72580
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GENBANK/Z18282
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GENBANK/Z18283