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. 1992;37(3):227-30.
doi: 10.1007/BF02933153.

Construction and Use of Agrobacterium Tumefaciens Binary Vectors With A. Tumefaciens C58 T-DNA Genes

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Construction and Use of Agrobacterium Tumefaciens Binary Vectors With A. Tumefaciens C58 T-DNA Genes

J Vlasák et al. Folia Microbiol (Praha). .

Abstract

Five plant morphoregulatory genes were isolated from the Agrobacterium tumefaciens Ti plasmid and binary plasmid vectors for plant transformation with these genes were constructed. All vectors have a similar structure with T-DNA borders, RK2 origin of replication and chimeric kanamycin resistance gene for the selection of transformed plant tissues. Over twenty vectors with single and combined morphoregulatory genes were constructed and their effects after tobacco tissue transformation studied.

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