Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material

Hepatology. 1992 Sep;16(3):682-7. doi: 10.1002/hep.1840160311.


The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax-embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochrome P-450 Enzyme System / classification
  • Cytochrome P-450 Enzyme System / genetics*
  • Endopeptidase K
  • Gene Expression
  • Humans
  • Liver / chemistry*
  • Liver / pathology
  • Liver Cirrhosis / genetics*
  • Liver Cirrhosis / pathology
  • Nucleic Acid Hybridization
  • RNA Probes
  • RNA, Messenger / analysis*
  • Serine Endopeptidases
  • Tissue Embedding


  • RNA Probes
  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Serine Endopeptidases
  • Endopeptidase K