The synovial proteome: analysis of fibroblast-like synoviocytes

Arthritis Res Ther. 2004;6(2):R161-8. doi: 10.1186/ar1153. Epub 2004 Feb 16.


The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial (FLS) cells derived from the synovia of rheumatoid arthritis patients. The cellular proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the in-gel digested proteins were analyzed by matrix-assisted laser desorption ionization mass spectrometry. A total of 368 spots were examined and 254 identifications were made. The studies identified a number of proteins that have been implicated in the normal or pathological FLS function (e.g. uridine diphosphoglucose dehydrogenase, galectin 1 and galectin 3) or that have been characterized as potential autoantigens in rheumatoid arthritis (e.g. BiP, colligin, HC gp-39). A novel uncharacterized protein product of chromosome 19 open reading frame 10 was also detected as an apparently major component of FLS cells. These results demonstrate the utility of high-content proteomic approaches in the analysis of FLS composition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Extracts / chemistry
  • Cells, Cultured
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Fibroblasts / chemistry*
  • Fibroblasts / metabolism*
  • Humans
  • Knee Joint
  • Proteome / analysis*
  • Proteomics / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Synovial Membrane / cytology*


  • Cell Extracts
  • Proteome