The genes for MHC class II regulatory factors RFX1 and RFX2 are located on the short arm of chromosome 19

Genomics. 1992 Aug;13(4):1307-10. doi: 10.1016/0888-7543(92)90052-t.

Abstract

RFX1 is a transacting DNA-binding regulatory factor involved in the control of MHC class II gene expression. RFX2 is a structurally very similar protein with identical DNA binding features. A member of the family of RFX factors is affected in an autosomal recessive disease, MHC class II deficient combined immunodeficiency (CID), caused by a defect in a trans-acting regulatory factor controlling MHC class II gene expression. In situ hybridization with 3H-labeled RFX1 cDNA has allowed us to identify two distinct targets on the short arm of chromosome 19 (19p13.1 and 19p13.2-p13.3). With the use of biotinylated genomic cosmid clones specific for RFX1 and RFX2, respectively, it was then possible to localize RFX1 at 19p13.1 and RFX2 at 19p13.2-p13.3. These two regulatory genes are thus assigned to a region of high gene density and RFX1 is close to another DNA-binding factor, LYL1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Chromosomes, Human, Pair 19*
  • DNA
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation
  • Genes, MHC Class II*
  • Humans
  • Karyotyping
  • Metaphase
  • Nucleic Acid Hybridization
  • Regulatory Factor X Transcription Factors
  • Regulatory Factor X1
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • DNA-Binding Proteins
  • RFX1 protein, human
  • Regulatory Factor X Transcription Factors
  • Regulatory Factor X1
  • Transcription Factors
  • DNA