Abstract
Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenine / chemistry*
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Bacillus / enzymology*
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Chromatography, Liquid
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Cytosine / chemistry*
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DNA Methylation
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DNA Modification Methylases / chemistry*
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DNA Modification Methylases / isolation & purification*
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DNA-Cytosine Methylases / chemistry*
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DNA-Cytosine Methylases / isolation & purification*
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Deoxyribonucleases, Type II Site-Specific / chemistry*
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry*
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / isolation & purification*
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Substrate Specificity
Substances
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Cytosine
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DNA Modification Methylases
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DNA-Cytosine Methylases
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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endodeoxyribonuclease Ksp632I
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Deoxyribonucleases, Type II Site-Specific
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Adenine