Isolation and characterization of site-specific DNA-methyltransferases from Bacillus coagulans K

Biochemistry (Mosc). 2004 Mar;69(3):299-305. doi: 10.1023/b:biry.0000022061.29918.8b.


Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / chemistry*
  • Bacillus / enzymology*
  • Chromatography, Liquid
  • Cytosine / chemistry*
  • DNA Methylation
  • DNA Modification Methylases / chemistry*
  • DNA Modification Methylases / isolation & purification*
  • DNA-Cytosine Methylases / chemistry*
  • DNA-Cytosine Methylases / isolation & purification*
  • Deoxyribonucleases, Type II Site-Specific / chemistry*
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry*
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / isolation & purification*
  • Substrate Specificity


  • Cytosine
  • DNA Modification Methylases
  • DNA-Cytosine Methylases
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • endodeoxyribonuclease Ksp632I
  • Deoxyribonucleases, Type II Site-Specific
  • Adenine