Disruption of the gene (spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A

FEMS Microbiol Lett. 2004 Apr 15;233(2):233-40. doi: 10.1016/j.femsle.2004.02.014.


This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Clostridium perfringens / genetics*
  • Clostridium perfringens / growth & development
  • Clostridium perfringens / metabolism
  • Enterotoxins / metabolism*
  • Gene Expression Regulation, Bacterial
  • Molecular Sequence Data
  • Mutagenesis
  • Plasmids
  • Spores, Bacterial / genetics*
  • Transcription Factors / genetics*


  • Bacterial Proteins
  • Enterotoxins
  • Spo0H protein, Bacillus subtilis
  • Transcription Factors
  • enterotoxin, Clostridium