Aurora B -TACC1 protein complex in cytokinesis

Oncogene. 2004 Jun 3;23(26):4516-22. doi: 10.1038/sj.onc.1207593.

Abstract

Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody - and consequently affects TACC1 localization at this site - and leads to abnormal cell division and multinucleated cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase
  • Aurora Kinase B
  • Aurora Kinases
  • Cell Division / physiology
  • Cell Nucleus / genetics
  • Fetal Proteins / genetics
  • Fetal Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Macromolecular Substances
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Mitosis / physiology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Transport
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA Interference
  • Spindle Apparatus / metabolism

Substances

  • Fetal Proteins
  • Macromolecular Substances
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • TACC1 protein, human
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases