Identification of murine and human XCP1 genes as C/EBP-epsilon-dependent members of FIZZ/Resistin gene family

Oncogene. 2004 Apr 22;23(19):3414-25. doi: 10.1038/sj.onc.1207126.


The CCAAT enhancer binding protein epsilon (C/EBP-epsilon) transcription factor is expressed predominantly in granulocytes. Mice with a disruption of the C/EBP-epsilon gene fail to produce mature granulocytes and eosinophils. Cells derived from the peritoneal exudates of C/EBP-epsilon -/- mice lack the expression of a number of chemokines and chemokine receptor genes. We have found a novel C/EBP-epsilon-dependent promyelocyte-specific gene, mXCP1. mXCP1 belongs to a family of XCP/FIZZ/Resistin genes, which includes four murine genes and two human genes, hXCP1 and hXCP2. These genes have four exons and encode short secreted proteins sharing a ten-cysteine motif. Murine mXCP1, mXCP2 and mXCP3 genes map to murine chromosome 16 and mXCP4 is positioned on chromosome 8; the hXCP1 and hXCP2 genes are located at homologous regions of chromosomes 3 and 19. Introduction of an inducible C/EBP-epsilon gene into the NIH3T3 and myeloid cells from C/EBP-epsilon-null mice line revealed that the conditional expression of C/EBP-epsilon induced mXCP1. The HXCP1 gene was identified as a C/EBP-epsilon-dependent regulatory homologue of mXCP1. The expression data for other members of XCP/FIZZ gene family are presented. Further studies indicate that XCP1 is a secreted protein that is chemotactic to myeloid cells from C/EBP-epsilon-null mice and is able to interact directly with alpha-defensin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins / physiology*
  • Chemotaxis
  • Chromosome Mapping
  • DNA, Complementary / analysis
  • Hormones, Ectopic / genetics*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Mice
  • Molecular Sequence Data
  • Nerve Growth Factor / genetics*
  • Organ Specificity
  • Proteins*
  • Resistin
  • alpha-Defensins / metabolism


  • CCAAT-Enhancer-Binding Proteins
  • Cebpe protein, mouse
  • DNA, Complementary
  • Hormones, Ectopic
  • Intercellular Signaling Peptides and Proteins
  • Proteins
  • RETN protein, human
  • RETNLB protein, human
  • Resistin
  • Retn protein, mouse
  • Retnla protein, mouse
  • alpha-Defensins
  • CEBPE protein, human
  • Nerve Growth Factor

Associated data

  • GENBANK/AF352730
  • GENBANK/AF352731
  • GENBANK/AF510097
  • GENBANK/AF510098
  • GENBANK/AF510099
  • GENBANK/BE847000