Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis

J Chromatogr A. 2004 Apr 2;1032(1-2):319-26. doi: 10.1016/j.chroma.2003.11.082.


Electrophoretically mediated microanalysis (EMMA) was applied for the study of the kinetic parameters of the enzymatic reaction of phenol sulfotransferase SULT1A1 isoenzyme with 4-nitrophenol as a substrate. The SULT1A1 activity was determined by the quantitation of the product, 4-nitrophenyl sulfate, at 274 nm by using different injection and separation steps. This new approach solved the problem of the presence of the very strong inhibitor, adenosine 3',5'-bisphosphate (PAP), in the co-substrate solution (adenosine 3'-phosphate 5'-phosphosulfate, PAPS) which is unstable at room temperature. The inhibitor PAP was electrophoretically separated from the co-substrate PAPS before the injection of enzyme and substrate inside the capillary (and thus before their in-capillary encountering). With the developed in-capillary SULT1A1 activity assay an average Michaelis constant (Km) for 4-nitrophenol was calculated to be 0.84 microM, a value which is consistent with a previously reported value. Strong substrate inhibition (above a 4-nitrophenol concentration of 2.5 microM) was observed, and this is also in accordance with literature values.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / pharmacology
  • Arylsulfotransferase / antagonists & inhibitors
  • Arylsulfotransferase / metabolism*
  • Enzyme Inhibitors / pharmacology
  • Kinetics


  • Enzyme Inhibitors
  • Adenosine Diphosphate
  • adenosine 3'-phosphate-5'-phosphate
  • Arylsulfotransferase