Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP

Mol Microbiol. 2004 Apr;52(2):437-49. doi: 10.1111/j.1365-2958.2004.03986.x.

Abstract

The chitobiose operon, chbBCARFG, encodes genes for the transport and degradation of the N-acetylglucosamine disaccharide, chitobiose. Chitobiose is transported by the phosphotransferase system (PTS) producing chitobiose-6P which is hydrolysed to GlcNAc-6P by the chbF gene product and then further degraded by the nagBA gene products. Expression of the chb operon is repressed by NagC, which regulates genes involved in amino sugar metabolism. The inducer for NagC is GlcNAc-6P. NagC binds to two sites separated by 115 bp and the transcription start point of the chb operon lies within the downstream NagC operator. In addition the chb operon encodes its own specific regulator, ChbR, an AraC-type dual repressor-activator, which binds to two direct repeats of 19 bp located between the two NagC sites. ChbR is necessary for transcription activation in the presence of chitobiose in vivo. Induction of the operon also requires CAP, which binds to a site upstream of the ChbR repeats. In the absence of chitobiose both NagC and ChbR act as repressors. Together these three factors cooperate in switching chb expression from the repressed to the activated state. The need for two specific inducing signals, one for ChbR to activate the expression of the operon and a second for NagC to relieve its repression, ensure that the chb operon is only induced when there is sufficient flux through the combined chb-nag metabolic pathway to activate expression of both the chb and nag operons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chitin / metabolism
  • Cyclic AMP Receptor Protein
  • DNA Footprinting
  • Disaccharides / metabolism*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Gene Expression Regulation, Bacterial*
  • Molecular Sequence Data
  • Mutation
  • Operon*
  • Plasmids
  • Promoter Regions, Genetic
  • RNA, Bacterial / isolation & purification
  • RNA, Bacterial / metabolism
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Recombination, Genetic
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Cyclic AMP Receptor Protein
  • Disaccharides
  • Escherichia coli Proteins
  • RNA, Bacterial
  • Receptors, Cell Surface
  • Repressor Proteins
  • Transcription Factors
  • crp protein, E coli
  • nagC protein, E coli
  • Chitin
  • chitobiose